Cryotechniques in Biological Electron Microscopy PDF Download. Download free ebook of Cryotechniques in Biological Electron Microscopy in PDF format or read online by Rudolf A. Steinbrecht,Karl Zierold 9783642728150 Published on 2012-12-06 by Springer Science & Business Media
To preserve tissue by freezing is an ancient concept going back pre sumably to the practice of ice-age hunters. At first glance, it seems as simple as it is attractive: the dynamics of life are frozen in, nothing is added and nothing withdrawn except thermal energy. Thus, the result should be more life-like than after poisoning, tan ning and drying a living cell as we may rudely call the conventional preparation of specimens for electron microscopy. Countless mishaps, however, have taught electron microscopists that cryotechniques too are neither simple nor necessarily more life-like in their outcome. Not too long ago, experts in cryotechniques strictly denied that a cell could truly be vitrified, i.e. that all the solutes and macro molecules could be fixed within non-crystalline, glass-like solid water without the dramatic shifts and segregation effects caused by crystallization. We now know that vitrification is indeed pos sible. Growing insight into the fundamentals of the physics of water and ice, as well as increasing experience of how to cool cells rapidly enough have enlivened the interest in cryofixation and pro duced a wealth of successful applications.
This Book was ranked at 16 by Google Books for keyword Macromolecules.
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Book which was published by Springer Science & Business Media since 2012-12-06 have ISBNs, ISBN 13 Code is 9783642728150 and ISBN 10 Code is 3642728154
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Download Cryotechniques in Biological Electron Microscopy PDF Free
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Download Cryotechniques in Biological Electron Microscopy PDF
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